MGnifams

A metagenomics-derived protein families resource

Protein Family: MGYF0000000014

Overview

This is the top-scoring MGnify protein (along with its specific region if not whole) that was recruited in the family through hmmsearch. Links to the MGnify Proteins site. Family representative sequence MGYP000895206080/4-137
# Amino Acids (AA) Representative length 134
The total number of MGnify sequences that have been iteratively recruited in the family through a series of processes such as: creating a seed alignment from the family's initial cluster, building an HMM model, and finally recruiting and aligning sequences from MGnify Proteins with the family HMM model. Total number of sequences in the family 380
Denotes if FunFam functional annotation hits were identified via the hmmer/hmmsearch tool. Sequence-HMM FunFam matches
Denotes if Pfam functional annotation hits were identified via the hmmer/hmmsearch tool. Sequence-HMM Pfam matches
Denotes if Pfam domain annotation hits were identified through model searching with the hhsuite/hhblits tool. Profile-profile Pfam matches
Denotes if structure homologs of the family's representative sequence have been identified in the AlphaFoldDB or PDB databases through the foldseek tool. Structure-structure hits

ESMFold structure

Predicted 3D protein structure through the Meta AI ESMFold model. ESMFold uses the representations from a large language model (ESM2) to generate an accurate structure prediction from the sequence of a protein.

For more information visit:

Download CIF file

  Very high (pLDDT ≥ 90)   High (90 > pLDDT ≥ 70)   Low (70 > pLDDT ≥ 50)   Very low (pLDDT < 50)

pLDDT corresponds to the model's prediction of its score on the per-residue Local Distance Difference Test. It is a measure of local accuracy. Confidence bands are used to colour-code the residues in the 3D viewer. The exact per-residue pLDDT value is shown when you mouseover the structure. Average structure plddt score: 61.3
The pTM score (predicted Template Modeling score) is a confidence metric that estimates how accurate the global topology of a predicted protein structure is likely to be. pTM score: 0.497

Predicted secondary structure The secondary structure prediction was carried out with the s4pred software. 

α-helices:  30.6%
β-strands:  33.58%
coils:      35.82%

The secondary structure shows a balanced composition of helices, strands, and coils, indicating a mixed fold.

Download features JSON file

Predicted transmembrane regions The transmembrane region prediction was carried out with the DeepTMHMMM software. 

inside:     74.63%
membrane-α: 20.9%
outside:    4.48%
signal:     0.0%
membrane-β: 0.0%
periplasm:  0.0%

This appears to be a transmembrane protein.

Download transmembrane JSON file

Multiple Sequence Alignment (Seed) This is the seed alignment that was used to create the HMM model of the family. It is different to the full alignment, which incorporates all MGnify sequences that have been recruited in the family after searching with the HMM model against the sequence pool. The full alignment is usually quite larger than the seed one and can be downloaded via the FTP.

Download seed MSA file

HMM viewer The family HMM is visualized via the Skylign API.

The height of each stack represents the information content (also known as relative entropy) at that position, while the size of each letter within the stack reflects its estimated probability. Click on a stack to highlight the corresponding column in the seed MSA viewer above.

Download HMM file

Biomes distribution An interactive sunburst plot showing the biomes where the family's underlying MGnify proteins were detected.

Download biomes CSV file

Domain architecture

The top 15 most prevalent domain architectures (including MGnifams and Pfams) found in the full alignment sequences of the family. The numbers on the left indicate how many MGnify sequences share each domain architecture.

Download domains JSON file

Functional annotation through Funfam matches

The family representative sequence was searched against the FunFam database (ver. 4.3.0) with hmmer/hmmsearch.

No FunFam hits found

Functional annotation through Pfam matches

The family representative sequence was searched against the Pfam database (ver. 38.0) with hmmer/hmmsearch.

Pfam Name E-value Score HMM from HMM to Alignment from Alignment to Envelope from Envelope to Accuracy
PF12158 DUF3592 7.1e-08 23.4 1 76 53 120 53 126 0.69

Profile-profile Pfam matches

This MGnifam HMM profile was searched against the HH-suite profile Pfam database (ver. 35.0) with HHsearch.

Pfam Name Description Probability E-value Length MGnifam HMM Pfam HMM
PF12158 DUF3592 Protein of unknown function (DUF3592) 97.9 7e-09 77 54-136 24-101 (129)

Structure-structure hits

This MGnifam 3D structure was searched against the Alphafold/UniProt and PDB databases with foldseek.

Rank Target Structure Target DB Aligned Length Query Start Query End Target Start Target End E-value
1 A0A0K1F0T4 AlphaFold 128 1 128 128 1.683e-16
2 A0A0K1F2W0 AlphaFold 128 1 128 128 2.884e-16
3 A0A0K1F255 AlphaFold 128 1 128 128 3.062e-16
4 A0A0K1F2T2 AlphaFold 131 1 131 131 5.57e-16
5 A0A3M1GPJ8 AlphaFold 128 1 122 145 1.384e-07
6 A0A0K1F2V6 AlphaFold 130 2 131 128 1.47e-07
7 A0A0K1F066 AlphaFold 133 2 131 136 4.065e-07
8 A0A395LW73 AlphaFold 145 3 122 156 1.815e-06
9 A0A845DFP9 AlphaFold 99 24 122 177 2.93e-06
10 A0A6B1G2T3 AlphaFold 99 24 122 177 6.008e-06
11 A0A3A4ZGA3 AlphaFold 131 6 122 132 6.008e-06
12 A0A845DIN7 AlphaFold 98 25 122 177 1.093e-05
13 A0A847FJL4 AlphaFold 116 7 122 116 1.308e-05
14 A0A1F2QYS6 AlphaFold 118 9 122 123 1.389e-05
15 A0A2E5VQ38 AlphaFold 145 3 122 473 1.474e-05
16 A0A1F2QNR1 AlphaFold 118 9 122 123 1.565e-05
17 A0A7W8J4C4 AlphaFold 116 23 122 126 1.565e-05
18 A0A366H897 AlphaFold 126 6 122 146 1.873e-05
19 A0A852VLS2 AlphaFold 134 23 134 144 2.111e-05
20 A0A081SGJ3 AlphaFold 146 2 121 156 2.848e-05
21 A0A1F2QHA0 AlphaFold 118 9 122 123 3.21e-05
22 A0A6C0TMS7 AlphaFold 106 23 122 112 4.597e-05
23 A0A417DII4 AlphaFold 108 24 131 130 5.501e-05
24 A0A7C2RKL2 AlphaFold 118 9 122 123 5.84e-05
25 A0A2V2WN05 AlphaFold 122 1 122 114 6.2e-05
26 A0A1I2CTZ7 AlphaFold 107 21 122 443 6.2e-05
27 A0A5B8XUC5 AlphaFold 152 4 134 457 6.2e-05
28 A0A2V9LMH3 AlphaFold 101 25 122 107 6.583e-05
29 A0A7H0M979 AlphaFold 119 4 122 116 6.583e-05
30 W9DYJ8 AlphaFold 124 6 122 142 6.989e-05
31 A0A1F8PQ37 AlphaFold 118 27 122 123 6.989e-05
32 A0A653TUE4 AlphaFold 117 6 122 110 7.42e-05
33 Q4CX01 AlphaFold 108 23 121 185 7.878e-05
34 A0A2W0BRI4 AlphaFold 119 8 122 120 8.363e-05
35 R5VLL1 AlphaFold 120 3 122 112 8.879e-05
36 A0A7G2C3P8 AlphaFold 120 3 122 114 8.879e-05
37 A0A1X0P871 AlphaFold 119 3 121 113 8.879e-05
38 A0A836GV04 AlphaFold 119 3 121 113 9.427e-05
39 A0A1X0NJD4 AlphaFold 143 13 122 147 0.0001001
40 A0A6L8T770 AlphaFold 99 25 123 123 0.0001063
41 A0A2W0BIL0 AlphaFold 121 2 122 120 0.0001063
42 A0A417DDX4 AlphaFold 82 41 122 125 0.0001128
43 A0A417CXL9 AlphaFold 108 24 131 131 0.0001128
44 V5B9Z4 AlphaFold 120 2 121 188 0.0001128
45 Q38B95 AlphaFold 119 3 121 113 0.0001198
46 A0A842C472 AlphaFold 134 2 122 142 0.0001198
47 A0A836HQC9 AlphaFold 119 3 121 113 0.000135
48 A0A0K2RB75 AlphaFold 95 33 122 153 0.000135
49 G0UW94 AlphaFold 119 3 121 113 0.000135
50 A0A837K4V6 AlphaFold 114 9 122 116 0.000135
51 A0A7Z2V4R2 AlphaFold 106 23 122 112 0.0001433
52 A0A3S0B590 AlphaFold 114 9 122 116 0.0001433
53 A0A7V8BQC0 AlphaFold 154 4 132 159 0.0001433
54 A0A2V9HAX3 AlphaFold 115 8 122 131 0.0001433
55 A0A849M1X4 AlphaFold 106 38 131 110 0.0001522
56 E9BTC8 AlphaFold 119 3 121 113 0.0001615
57 A0A061JBA3 AlphaFold 122 1 122 114 0.0001821
58 A0A640KV46 AlphaFold 119 3 121 113 0.0001821
59 A0A0H3G0V8 AlphaFold 114 9 122 116 0.0001821
60 A0A2V9AXX0 AlphaFold 115 8 122 131 0.0001821
61 A0A5C9EQ60 AlphaFold 122 1 122 113 0.0001821
62 A0A549SCG1 AlphaFold 85 37 121 97 0.0001933
63 D0A2N3 AlphaFold 119 3 121 113 0.0001933
64 A0A7L9BKZ6 AlphaFold 117 6 122 169 0.0002052
65 E9ASI3 AlphaFold 119 3 121 113 0.0002052
66 A0A7K3LWK0 AlphaFold 119 3 121 125 0.0002052
67 A0A2W0BBZ2 AlphaFold 105 41 134 109 0.0002179
68 Q4CXM7 AlphaFold 117 16 121 184 0.0002179
69 A0A3L6L3R7 AlphaFold 119 3 121 113 0.0002179
70 A0A3S7XAC1 AlphaFold 119 3 121 113 0.0002179
71 A0A506XT11 AlphaFold 112 20 121 168 0.0002179
72 A0A1Q7F4T5 AlphaFold 118 22 122 118 0.0002313
73 A0A0K1EZW9 AlphaFold 135 2 131 140 0.0002313
74 A0A417DDX6 AlphaFold 122 2 123 123 0.0002313
75 A0A7J6Y900 AlphaFold 120 3 122 186 0.0002313
76 A0A2V9MN51 AlphaFold 118 19 122 136 0.0002313
77 A0A837JMW4 AlphaFold 114 9 122 116 0.0002313
78 A0A6M1QXF9 AlphaFold 88 36 122 135 0.0002456
79 A0A417DI59 AlphaFold 120 4 123 120 0.0002456
80 A0A2V9ZD80 AlphaFold 121 2 122 118 0.0002456
81 A0A422P2C4 AlphaFold 122 1 122 114 0.0002456
82 A0A842GDD0 AlphaFold 134 2 122 142 0.0002456
83 A0A842RNT9 AlphaFold 118 5 122 114 0.0002456
84 A0A7X1DIL2 AlphaFold 134 2 122 142 0.0002456
85 A0A3D8MVW8 AlphaFold 133 2 127 460 0.0002608
86 A0A852Q1W5 AlphaFold 97 27 122 116 0.0002608
87 A0A321LTP6 AlphaFold 120 21 122 120 0.0002608
88 A0A7Y9LS97 AlphaFold 120 3 122 144 0.0002608
89 A0A1Y4D875 AlphaFold 126 1 122 152 0.0002768
90 A0A836I7D5 AlphaFold 119 3 121 113 0.0002768
91 A0A7C2TAJ6 AlphaFold 125 20 122 128 0.0002768
92 A0A3M2FP24 AlphaFold 145 1 122 145 0.0002768
93 A0A7V4U2U1 AlphaFold 141 2 121 143 0.0002939
94 A0A2V9SJB7 AlphaFold 119 4 122 118 0.0002939
95 A0A1G4I9V0 AlphaFold 119 3 121 113 0.0002939
96 A0A841XI83 AlphaFold 134 2 122 142 0.0002939
97 A0A841XVP9 AlphaFold 134 2 122 142 0.0002939
98 A0A7X0YL44 AlphaFold 134 2 122 142 0.0002939
99 A0A3S2A499 AlphaFold 98 24 121 101 0.000312
100 A0A3Q8Z8B2 AlphaFold 98 24 121 101 0.000312
101 A0A417PKR7 AlphaFold 99 25 123 120 0.000312
102 A0A2V9UQC6 AlphaFold 120 22 122 120 0.000312
103 A0A431L8J2 AlphaFold 109 22 122 211 0.000312
104 A0A2S9D597 AlphaFold 117 20 128 119 0.000312
105 A0A7X0Z121 AlphaFold 134 2 122 142 0.000312
106 A0A1C5VBE7 AlphaFold 123 22 122 123 0.000312
107 A0A2G9SNM3 AlphaFold 145 2 122 155 0.0003313
108 A0A7G8SME6 AlphaFold 114 9 122 115 0.0003313
109 A0A842GHX6 AlphaFold 134 2 122 142 0.0003313
110 A0A5C6UTX9 AlphaFold 131 4 127 460 0.0003313
111 A0A239LCN9 AlphaFold 94 35 122 101 0.0003517
112 A0A417PKK7 AlphaFold 120 4 123 126 0.0003517
113 A0A2V9XEG2 AlphaFold 115 8 122 118 0.0003517
114 A0A417CXV2 AlphaFold 121 3 123 120 0.0003517
115 A0A417DIA6 AlphaFold 97 27 123 151 0.0003734
116 A0A836GDN1 AlphaFold 119 3 121 113 0.0003734
117 A0A417DDU8 AlphaFold 120 4 123 120 0.0003734
118 A0A842W022 AlphaFold 117 6 122 112 0.0003734
119 A0A177PLG4 AlphaFold 85 37 121 97 0.0003964
120 A0A439HMJ2 AlphaFold 98 24 121 101 0.0003964
121 A0A1Y4D1R2 AlphaFold 127 2 122 152 0.0003964
122 A0A399RQS9 AlphaFold 103 21 122 105 0.0003964
123 A0A251ZEU8 AlphaFold 97 27 122 116 0.0003964
124 A0A842E592 AlphaFold 134 2 122 142 0.0003964
125 A0A7X0ZXE1 AlphaFold 134 2 122 142 0.0003964
126 A0A842D9J6 AlphaFold 134 2 122 142 0.0003964
127 A0A0K2NWX2 AlphaFold 116 21 122 116 0.0003964
128 A0A1V5YC07 AlphaFold 103 20 122 113 0.0004209
129 A0A088S1F8 AlphaFold 119 3 121 113 0.0004209
130 A0A0M9CBA0 AlphaFold 119 3 121 125 0.0004209
131 A0A1F5UJQ1 AlphaFold 128 1 122 157 0.0004209
132 K3W461 AlphaFold 126 2 122 154 0.0004209
133 A0A328CBN9 AlphaFold 133 3 127 461 0.0004209
134 A0A495P1L3 AlphaFold 117 6 122 111 0.0004468
135 A0A7X1A542 AlphaFold 134 2 122 142 0.0004468
136 A0A436RPJ8 AlphaFold 98 24 121 101 0.0004744
137 A0A0D6SW78 AlphaFold 81 50 122 109 0.0004744
138 A0A2N0GF07 AlphaFold 96 27 122 110 0.0004744
139 A0A0N0DV63 AlphaFold 126 3 128 122 0.0004744
140 A0A3P3ZIH1 AlphaFold 127 2 128 123 0.0004744
141 A0A7X1BV47 AlphaFold 134 2 122 142 0.0004744
142 A0A842TD54 AlphaFold 121 2 122 114 0.0004744
143 A0A444K7K0 AlphaFold 98 24 121 101 0.0005037
144 A0A1B1Y3F9 AlphaFold 101 22 122 94 0.0005037
145 A0A7I0KAT5 AlphaFold 95 27 121 101 0.0005037
146 A0A5C7Z8P4 AlphaFold 115 15 122 212 0.0005037
147 A0A519T780 AlphaFold 109 21 128 110 0.0005037
148 A0A0F9TLT7 AlphaFold 112 20 121 137 0.0005037
149 A0A2V9UPJ1 AlphaFold 119 21 122 120 0.0005037
150 A0A2V2VS79 AlphaFold 119 3 121 113 0.0005037
151 A0A679GKQ6 AlphaFold 102 37 128 117 0.0005037
152 A0A1Q7MRK9 AlphaFold 119 4 122 118 0.0005037
153 A0A2V9K2G1 AlphaFold 121 2 122 133 0.0005037
154 A0A836GBB7 AlphaFold 119 3 121 113 0.0005037
155 A0A417DE27 AlphaFold 122 2 123 123 0.0005347
156 A0A6S5RX55 AlphaFold 81 50 122 109 0.0005347
157 A0A504X274 AlphaFold 122 1 122 114 0.0005347
158 A0A5C2HKP0 AlphaFold 148 3 134 271 0.0005347
159 A0A842CKZ8 AlphaFold 134 2 122 142 0.0005347
160 A0A7W1LXM6 AlphaFold 82 41 122 95 0.0006027
161 A0A0D6TKE5 AlphaFold 96 27 122 103 0.0006027
162 A0A1Q6N8S0 AlphaFold 129 3 131 125 0.0006027
163 A0A521THL5 AlphaFold 127 7 133 140 0.0006027
164 A0A1J1CCW8 AlphaFold 121 5 122 174 0.0006399
165 A0A855X594 AlphaFold 121 4 122 150 0.0006399
166 A0A1I6U586 AlphaFold 98 25 122 110 0.0006399
167 A4ICS1 AlphaFold 119 3 121 113 0.0006399
168 A0A2V9VGV8 AlphaFold 119 4 122 118 0.0006399
169 A0A2V9QKH4 AlphaFold 119 4 122 118 0.0006399
170 A0A2V9R7E8 AlphaFold 119 4 122 119 0.0006399
171 A0A2V9ZC29 AlphaFold 122 21 122 125 0.0006399
172 A0A6L8TA83 AlphaFold 119 4 122 119 0.0006399
173 A0A6I3ES67 AlphaFold 119 21 122 125 0.0006399
174 A0A2V9TVM4 AlphaFold 125 21 134 125 0.0006399
175 A0A841HVQ2 AlphaFold 109 22 122 112 0.0006399
176 A0A1I5SFL3 AlphaFold 106 26 131 235 0.0006794
177 A0A2V9QEQ8 AlphaFold 120 3 122 117 0.0006794
178 A0A2V9U8M4 AlphaFold 119 4 122 118 0.0006794
179 A0A1M5TWH9 AlphaFold 108 20 121 120 0.0006794
180 A0A842ENR1 AlphaFold 131 2 122 142 0.0006794
181 A0A0D7VW25 AlphaFold 98 25 122 108 0.0007213
182 A0A5C6YZP8 AlphaFold 103 20 122 100 0.0007213
183 A0A2N9LX85 AlphaFold 119 4 122 118 0.0007213
184 A0A2V9YAP7 AlphaFold 119 4 122 135 0.0007213
185 A0A1Q7CG15 AlphaFold 119 4 122 118 0.0007213
186 A0A353R143 AlphaFold 120 20 122 124 0.0007213
187 A0A3S0ELJ3 AlphaFold 104 21 122 211 0.0007657
188 A0A6L9J192 AlphaFold 127 3 129 157 0.0007657
189 A0A068NVR0 AlphaFold 111 27 132 257 0.0007657
190 A0A261FN51 AlphaFold 118 24 122 122 0.0007657
191 A0A7H0M984 AlphaFold 100 27 122 116 0.0007657
192 A0A7C6E5F2 AlphaFold 93 37 129 133 0.000813
193 A0A3B8SQ17 AlphaFold 118 5 122 99 0.000813
194 A0A352SJB7 AlphaFold 120 3 122 115 0.000813
195 R7C5I2 AlphaFold 120 3 122 115 0.000813
196 A0A1Q7V7A9 AlphaFold 121 2 122 118 0.000813
197 A0A495FMN3 AlphaFold 120 3 122 133 0.000813
198 A0A1E1J740 AlphaFold 127 2 128 123 0.000813
199 A0A1Y4FM83 AlphaFold 136 1 132 162 0.0008631
200 A0A1V3C5D4 AlphaFold 110 21 122 327 0.0008631
201 A0A3R6NXV2 AlphaFold 120 3 122 115 0.0009163
202 A0A101BHX4 AlphaFold 127 2 128 139 0.0009163
203 A0A351WPC1 AlphaFold 110 13 122 107 0.0009163
204 A0A442B799 AlphaFold 111 20 121 152 0.0009163
205 A0A7X0XIU3 AlphaFold 131 2 122 142 0.0009163
206 A0A4S3LZN5 AlphaFold 105 22 122 107 0.0009729
207 A0A535EXF0 AlphaFold 121 2 122 118 0.0009729
208 A0A8B3P3V2 AlphaFold 114 8 121 150 0.0009729
209 A0A2U3KGQ7 AlphaFold 128 2 122 133 0.0009729
210 A0A2D6AEL1 AlphaFold 126 6 131 142 0.0009729
211 A0A524D256 AlphaFold 120 2 121 112 0.0009729
212 A0A841VY90 AlphaFold 134 2 122 142 0.0009729
213 A0A842FEV4 AlphaFold 131 2 122 142 0.0009729

Family Representative Sequence Viewer

Amino acid position: -

ALLALYGWFSLVLYVIFAVAPRSSSAVAMALWSALSLALSAHIAWRRLHWVPVEGEVVEVRSGGPGSGSWSFGWRWSYGGVTHTAADVIGEWFVRFALLFNRIGKGTRATLWVDPRDPSRLLPPTWRAPLLLPL

HMM Consensus aLlalyvvftllllvlialkgrselglalalllaaalslalaahiawrrlrtvpVegelvdvereeapsykrrryrswtpvwrYtygGrtytvedtipsnklveigrrgtlwVDPrdPsrllppswrerllvyllfaalcllvaall